Papers

PAPERS IN LEARNED JOURNALS

S/N Author (s) Date Title Name of Journal Edition Page Number Link/URL
(If applicable)
Upload Abstract
(pdf)
1 Bem, A. A.1, Ogwu, I. M.2, Terna, T. P.1, Waya, J. I.2, Amua, Q. M.3 and Orpin, J. B.1 2012-11-30 Preliminary Screening of Antimicrobial Activity of Some Selected Plant Extracts on a Clinical Sample of Staphylococcus aureus Esxon Publishers 12 56-60 A comparative evaluation of the antimicrobial activity of ethanolic extracts of three ethno-medicinal plants on a clinical sample of Staphylococcus aureus was conducted in Botany Laboratory of the Department of Biological Sciences, Benue State University, in 2012. Leaves of Chromolaena odorata, Irvingia gabonensis and fruits of Piper nigrum were collected in the wild, air dried and grounded into powder form. The extraction of compounds and phytochemical screening to determine active ingredients was by Soxhlet’s extraction methods. Antimicrobial activities of the extracts were evaluated using the plate dilution method at concentrations ranging from 0.5mg/ml-2.0mg/ml ethanolic extract relative to control. Results showed presence of alkaloids and flavonoids in all the three plants. Saponins were completely absent in Piper nigrum but contained steroids with tannins completely absent in Chromolaena odorata. Test organism was sensitive to the three plant extracts. Relative mean colony count of test organism ranged from 4.00-59.0 after treatment with different doses of plant extracts with significant difference (P = 0.05) at minimum concentration of 2.0mg/ml. At the concentration of 2.0mg/ml, extracts of Piper nigrum had higher antimicrobial activity on the test organism. Key words: Antimicrobial activity, Plant extracts, Staphylococcus aureus
2 Orpin, J.B 2013-08-24 Identification and Studies of some common dermatophyte infections in Makurdi Benue State BEST 5 49-58 Upload
3 Orpin, J. B.1, Olusi, T. A.2, Bem, A. A.1, Akaahan, T. J.3, Terna, P.1, Anum, T.4 and Obaje, M. O.1 2013-01-31 Co-Infection of Leishmania and Human Immunodeficiency Virus (HIV) Among Patients Attending Gboko Health Division, Benue State, Nigeria ESXON 13 40-46 Studies on co-infection of Leishmania and Human immunodeficiency virus (HIV) were carried out at Gboko Health Division, Benue State from August 2008- July 2009. One thousand patients were sampled at random; Leucoconcentration method was used in the diagnosis of Leishmania while commercially procured HIV test kits (Chembio HIV I & II STAT PAK) were used in screening for HIV seropositivity. Results showed that 66 (6.6%) of the patients sampled were found coinfected with Leishmania and HIV, 62 (6.2%) were positive for visceral leishmaniasis single infection while 308 (30.8%) were positive for HIV infection alone. Infection rates of Leishmania, HIV and of both pathogens were significantly different (p<0.05) based on sex and age using the chi-squared analysis. Male patients sampled were infected more by HIV than their female counterparts with percentage of 55.14% in the age group 21-30 years and 46.67% in females within the age group 31-40 years; whereas females were more infected by Leishmania with 19.57% in the age group 41-50 years and 15.38% for males in the age group 11-20 years. The presence of co-infection of Leishmania and HIV in the study area is of clinical importance and requires routine, constant check and public health awareness. Keywords: Co-infection, Leishmania, HIV, Patients, Benue State.
4 Bem, A. A.1, Oluma, H. A. O.2, Nwankiti, A. O.2, Terna, T. P.1, Waya, J. I.1, Olutade, A. O.1, Gwa, V.3 and Orpin, J. B.1 2013-01-31 Effects of Artificial Inoculation of Pathogenic Strains of F. oxysporum And Sclerotium rolfsii on some selected varieties of Tomato (Lycopersicon esculentum). ESXON 12 40-45 A study to evaluate the effect of some fungal pathogens on the yield of selected local and improved Tomato (Lycopersicon esculentum) varieties was carried out by artificially inoculating Plants grown in polythene bags containing sterilized organic soils with pathogen inoculum. Yield parameters were obtained by measuring stem heights (cm), number of leaves, flowers and fruits. Yield loses were calculated in percentages. Slerotium rolfsii was most devastating with values of reductions in yield ranging from 0.00 in Roma VFL, Peto VFL, UC Giant and Local variety, to 3.00 in Romaking. The local variety was most susceptible to S. rolfsii and F. oxysporum with respect to yield (0.00 respectively). Romaking was most tolerant to S. rolfsii (3.00 fruits) while Peto VFL was most tolerant to F. oxysporum (19.00 fruits). The effect of synergistic activity of both pathogens to yield was most devastating on UC Giant (0.00 fruits) and least on Romaking (14.0 fruits). Effects of separate inoculation of pathogens on different yield parameters of different tomato varieties were statistically significant (P≤0.05). Their synergistic effect was not statistically significant (P≤0.05).
5 Bem, A.A.1 Terna, T.P.1, Iyoula, F.I.2, Waya, J.I.2, Orpin, J.B.1 and Manir, N.1 2013-04-01 Preliminary Studies On Production And Partial Purification Of Toxins Associated With Black Tar Disease Of Yam (Dioscorea Species) In Makurdi, Benue State, Nigeria. IOSR Journal Of Environmental Science, Toxicology And Food Technology (IOSR-JESTFT) 4 10-15 www.Iosrjournals.Org Studies were carried out to identify pathogens and toxins associated with the black tar disease of yam foliage (Yam anthracnose) in Makurdi, Benue State, Nigeria. Infected yam leaves and stems showing symptoms were collected from farms and cultured for isolation of causal organisms. Pure cultures of isolates were obtained using single spore method. Toxin extractions were done using 100ml of ethyl acetate in separating funnels. These were dried using a vacuum rotator evaporator. Partial purification of the toxin was done with the use of thin layer chromatography (TLC) plates. Results showed the predominant presence of Colletotrichum gloeosporioides. TLC quantification in four solvent systems suggests that the toxin produced is a glycoprotein. Keywords: Black tar disease of yam, Colletotrichum gloeosporioides, Glycoprotein, TLC (Thin Layer Chromatography), Toxins.
6 Liamngee K1., Terna T.P2., Bem A.A2., Orpin J.B2., Mzungu I2., Obaje M2. and Anum, T3 2013-04-01 Microbial Analysis of Soyabean Milk Sold In Makurdi Metropolis IOSR Journal Of Environmental Science, Toxicology And Food Technology (IOSR-JESTFT) 13 11-16 www.Iosrjournals.Org A survey of the production and consumption rate of soymilk within Makurdi metropolis and its microbial quality was carried out. Samples were collected from women and children who hawked the product in Northbank, Wurukum, Highlevel and Wadata areas of Makurdi. Data on soymilk production and consumption was collected using questionnaire method. Pour plate method and relevant biochemical tests were used for isolation, identification and characterisation of microbes. Proximate analysis was carried out to determine the nutrient content of milk samples. Producer educational status showed that 54.55% of the producers had primary school qualification while 27.27% had secondary school certificates; 18.18% were graduates. The consumption rate showed that 61.79% were daily consumers, 31.07% occasional consumers and 7.14% made up non-consumers. Proximate chemical analysis showed that the samples had high moisture content ranging from 68.50 – 91.49% with the ash content being 0.30 – 0.90%. The fibre content ranged from 0.10% – 0.35% and the lipid content, 1.56% – 17.10%. The protein content ranged from 2.86 to 7.76% while the carbohydrate content ranged from 3.09 – 13.93%. The microbial load of soymilk ranged from 6.9 × 107 – 7.6 × 107 c.f.u./ml for North bank, 4.1 × 107 – 5.6 × 107 c.f.u./ml for Wurukum, 3.0 × 107 – 4.7 × 107 c.f.u./ml for High level and 6.0 × 107 – 8.5 × 107 c.f.u./ml for Wadata respectively. All samples were contaminated with members of the enterobacteriaceae, including E. coli, Klebsiella spp, Salmonella typhi, Streptococcus faecalis, and Staphylococcus aureus. Soymilk sold in the sampled areas were highly contaminated with faecal microbes, indicating poor hygiene of handlers and sanitary quality of processing water. Key Words: Biochemical test, Makurdi, microbial load, proximate analysis, soymilk,
7 Bem, A.A1, Antsa, R.T.2, Orpin, J.B1, Bem, S.L2, And Amua, Q.M1 2014-07-01 Root-Knot Nematode (Meloidogyne Species) Distribution in Some Tomato Fields in Makurdi IOSR Journal Of Environmental Science, Toxicology And Food Technology (IOSR-JESTFT) 12 1-7 www.Iosrjournals.Org Studies were conducted to assess the distribution of root-knot nematode in some tomato fields in Makurdi from October 2010 to January 2011. Tomato plants were surveyed for infection based on symptoms such as stunted growth, yellowing of leaves and wilting in farmers’ farm for determining disease incidence. Number of knots (NK) and root knot index (RKI) was used for disease severity. Disease plant Samples were collected and taken to the laboratory for extraction. Perineal patterns were prepared and examined under the microscope and species identified using appropriate keys. The result shows incidence range from 20%-60% in October, 2010 and 20%-80% January, 2011 while the severity of the disease ranged from 1-3 in October, 2010 and 1-4 in January, 2011. Significant differences (P=0.05) in the study areas. Egg Mass Index (EMI) and Gall Index (GI) ranged from 1.0 – 4.0. Meloidogyne incognita and M. javanica were identified to be the causes of infection. It is suggested that control methods should be applied in the nematode management in farmers fields so as reduce nematode population and increase yield. Key words: Disease symptoms, Root Knot, Perineal Pattern, Meloidogyne, Tomato
8 Anum, T1 , Orpin, J. B2, Bem, A.A2 , Mzungu, I2, Aliyu, Y2 2014-07-01 Human Water Contact Behaviour and Schistosoma haematobium Infection among Primary School Pupils in Guma LGA of Benue State. IOSR Journal Of Environmental Science, Toxicology And Food Technology (IOSR-JESTFT) 11 10-16 www.Iosrjournals.Org A study was carried out to investigate “Human water-contact and the transmission of S. haematobium infection among Primary School pupils in Guma Local Government Area of Benue State”. Parasitological screening was carried out on 643 pupils, 455 (70.8%) males and 188 (29.2%) females. Out of the total figure, 352 tested positive [252 (55.4%) males and 100 (53.2%)] for the infection of S.haematobium. There was also a significant difference in the prevalence rate in the male and female population with the males having higher infection than females, (X2 cal=6.61 > X2 crit. = 5.99 at P≤ 0.05 and 2df). The prevalence of the infection was significantly higher in Yelwatta (68.0%) than Gbajimba (51.1%) and Daudu (50.4%), (X2cal = X2crit. =5.99 at P ≤ 0.05 and 2 df). The intensity of infection on the other hand was significantly higher in Daudu (39.2%) than Gbajimba (31.8%) and Yelwatta (29.0%), (X2 cal =11.83 > X2crit. =5.99 at P ≤ 0.05 and 2df). Individuals aged 6-10 years old were the most infected, and the intensity of infection was higher among males. The demographical survey data also revealed that pupils who had parents as farmers and fishermen were more exposed to the infection of S.haematobium (43.0% vs 30.4%). To control urinary schistosomiasis, methodologies and managerial tools should be integrated to improve preventive strategies with emphasis on health education, information and communication. Key words: Human, Water Contact, Behaviour and S.haematobium